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Dietary fibre (DF) is a beneficial part of healthy diet. DF is fermented and utilised by intestinal microbiome, supporting the diversity of microbial community, and it delivers phytochemicals to colon for conversion to easily accessible products, which enter circulation. The “hype” around intestinal microbiome has increased the development of methods, which enable systematic evaluation of DF ingredients in capacities to modulate microbiome and to produce components with potential promotion of intestinal and systemic health.